| 초록 |
We designed the thermo-responsive carbon dots for cancer cell imaging via carbonization of poly(N-isopropylacrylamide) (PNIPAAm). We compared between two pathways of carbonization via acid treatment: partially (CD(PNIPAAm)) and fully carbonized (CD) materials based on the carbonization time. The CD(PNIPAAm) showed lower critical solution temperature (LCST) behavior and reversible fluorescent intensity depend on temperature, with a high fluorescent signal observed at room temperature (25ºC) but quenching at physiological temperature (37ºC), enabling the reversible fluorescent on-off platform. This material demonstrated excellent biocompatibility and in vitro bioimaging using MDAMB, A-549, and MDCK cells. This CD(PNIPAAm) exhibited tunable fluorescent properties, high aqueous stability, and low cytotoxicity, suggesting potential applications in bio-labeling, bio-imaging, and optoelectronics. This work was supported by Korea National University of Transportation in 2021. |
