| 초록 |
To achieve effective bone regeneration, the structural and biological environment must be considered. Here, we investigated the influence of structure of electrospun PLLA fibrous scaffolds and incorporated growth factors (BMP-2 and PDGF) on in vitro differentiation of human mesenchymal stem cells (hMSCs) cultured onto the scaffolds. GFs were encapsulated into coacervate (Coa). Coa-coated nano-sized PLLA (Coa-nPLLA) exhibited relatively higher coating efficacy, than Coa-coated micro-sized PLLA (Coa-mPLLA) due to large mesh hole area of mPLLA. Consequently, a faster GF release pattern was observed in nPLLA groups. As a result of osteogenic differentiation of hMSC which cultured onto GFs loaded Coa-PLLA, the architectural cue rffectively modulated early osteogenic differentiation of hMSCs. Facilitated long-term differentiation of hMSCs could be achieved by synergistic effect of dual BMP2 and PDGF in nanofbrous environments. |
