| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.388 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Preparation of PHBV-collagen nanofiber scaffolds by electrospinning and their interaction with fibroblasts |
| 초록 | Introdution Poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid)s (PHBVs) are highly crystalline polymers with melting points and glass transition temperature similar to polypropylene. Owing to the characteristics of biodegradability and easy processability, PHBVs are being developed and commercialized as ideal candidater for the substitution of non-biodegradable polymeric materials in commodity applications1. Collagen is a biodegradable, biocompatible, and non-immunogenic structural protein, which makes it a suitable compound for a variety of biomedical application2. Recently, much attention has been paid to electrospinning process as a unique technique because it can produce nanofiber scaffolds with diameter in the range from several micrometer down to tens nanometers. With respect to surface microstructure, nanofibrous scaffolds are the most promising materials used in various tissue engineering applications because of their high specific surface area and highly porous three dimensional structure. In this study, PHBV and collagen typeⅠwere used to fabricate electrospun nanofiber scaffold. Characteristics of nanofiber scaffolds were investigated using ATR-FTIR spectroscopy and electron spectroscopy for chemical analysis. Behaviors of fibroblasts on nanofiber scaffolds were also investigated. Results and Discussion The SEM micrograph of nanofibers electrospun from the PHBV/collagen solution was shown in Figure 1. Interestingly, PHBV nanofibers with diameters ranging from 0.8∼1.2㎛ were formed and collagen nanofibers with diameter smaller than 100 nm were netted between PHBV fibers. Figure 2 shows the fibroblasts grown on the fiber mat for 1 day. The SEM micrographs showed that the fibroblasts adhered, spread, and proliferated on the surface of PHBV-collagen nonwoven mat. This results suggest that PHBV-collagen nanofiber mat can provide a good micro-environment for the cells attachment and proliferation. Fig. 1. A view of PHBV/collagen nanofiber mat. Fig. 2. SEM view of the fibroblasts grown on the nanofiber mat for 24h. References 1. M. Avella, B. Immirzi, M. Malinconico, E. Martuscelli, and M. G. Volpe, Polym. Int., 39, 191 (1996). 2. L. Huang, K. Nagapudi, R. P. Apkarian, and E. L. Chaikof, J Biomater Sci. Polym Edn, 12, 979 (2001). |
| 저자 | 맹 만, 강인규 |
| 소속 | 경북대 |
| 키워드 | PHBV; collagen; nanofiber; electrospun |
