| 초록 |
Folic Acid (FA) is one of most widely utilized targeting ligands for drug delivery systems (DDS). Folate receptors (FR) are abundantly expressed on many types of tumor cells. Therefore, FA can be used for targeted delivery systems for genetic materials such as pDNA and siRNA. However, many studies revealed that the endosomal release of cargo drugs was critical bottleneck for FA-mediated DDS, requiring additional use of the endosomal releasing agents. In this study, we have synthesized FA immobilized AuNRs for the delivery of siRNAs in target cells. AuNRs is known to induce excellent photothermal effect, when treated with a NIR laser. In the study, we have first synthesized the AuNRs with PEG-amine. HS-PEG-NH2 is added for generating stable PEG coating on AuNRs. The PEG-coated gold nanorods (AuNRs-PEG) are consequently reacted with the SPDP and siRNA, which is modified with –thiol. With AuNR-siRNA conjugates, we can expect to achieve effective endosomal releasing under laser stimulation and photo-triggered gene silencing can be verified in the green fluorescence protein (GFP) expressing KB cells. |
