| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.25 |
| 발표분야 | 의료용 주입형 하이드로젤 |
| 제목 | In Vivo Chondrogenesis using Human Umbilical Cord Blood derived Mesenchymal Stem Cell and Injectable Hydrogel Polymer |
| 초록 | Mesenchymal stem cells(MSCs) are known to have the potential to differentiate into chondrogenic, osteogenic and adipogenic cells. We developed a novel method to isolate and culture-expand MSC populations from human umbilical cord blood. The characterization of the MSC population was performed by verificationof the MSC surface antigen using FACS. The MSC population achieved by our technique was successfully differentiated into chondrogenic, osteogenic and adipogenic lineage. The articular cartilage per se is still known to be irreparable and hard to be regenerated in vitro or in vivo by currently available techniques. In this study, in vivo articular cartilage regeneration was investigated using biodegradable injectable hydrogel polymer. Critical size full-thickness articular cartilage defects was created in the knee joint of New Zeland White Rabbit, and the defects were filled with a mixture of the human umbilical cord blood derived MSCs and hydrogel polymer. Tissue samples were harvested at 8 weeks and 16 weeks after the implantation. Gross and microscopic findings were evaluated and biomechanical study was performed. White and glistening articular cartilage-like tissues were more frequently observed in theMSC-hydrogel mixture group than in the defect only or hydrogel only group. Microscopic finding with various staining methods revealed the evidence of articular cartilage regeneration by the MSC-hydrogel mixture. Biomechanical study revealed reasonable strength of the repaired cartilage defect area. The injectable hydrogel polymer seems to be a valuable vehicle for articular cartilage repair by human umbilical cord blood derived mesenchymal stem cells. |
| 저자 | 김지형1, 양성은2, 양윤선3, 하철원3 |
| 소속 | 1성균관의대 삼성서울병원 정형외과, 2임상의학(연), 3메디포스트㈜ 생명공학(연) |
| 키워드 | Chondrogenesis; Mesenchymal stem cell; Hydrogel polymer |
