| 초록 |
We prepared a temperature-responsive culture dish grafted with a poly(N-vinylcaprolactam-co-acrylic acid) (PNVCL-co-PAAc) by electron beam irradiation. Various types of cells adhere, spread and proliferate on the grafted tissue culture polystyrene (TCPS) dishes in the presence of serum at 37℃. Rapid detachment of the cultured cell sheets is a very important recovery method that permits a facile manipulation of the sheet and prevents functional damage. By reducing only the temperature, theses cells can be harvested noninvasively from the dishes according to the rapid hydration of the grafted polymer. Because the harvest does not need enzymatic digestion, differentiated cell phenotypes are retained. In this study, a human fibroblast was cultured on such dishes and its cell behavior was examined. Analyses by ATR-FTIR, AFM and ESCA revealed that the PNVCL-co-PAAc was successfully grafted to surfaces of the TCPS dishes. |
