| 초록 |
A large-scale production of DNA nanostructures for siRNA delivery has been achieved by an isothermal enzymatic process of rolling circle amplification (RCA). Our system allowed the preparation of more than 200-fold amplified DNA products using ss circular DNA template. Y-shaped DNA nanostructures with sticky overhangs, which can hybridize with siRNAs, were generated through the programmed molecular self-assembly. The DNA nanostructures loaded with folate conjugated-siRNAs showed enhanced cellular uptake as well as dose-dependent gene silencing. |
