| 초록 |
Methanotrophs play an important role in biotechnological applications, with their ability to utilize single carbon (C1) feedstock such as methane and methanol to produce a range of high-value compounds. A novel obligate methanotroph, Methylomonas sp. DH-1, became a platform strain for biotechnological applications because it is capable of producing chemicals, fuels, and secondary metabolites from methane and methanol. The genome of Methylomonas sp. DH-1 consists of one 4.86 Mb chromosome and one 278 kb plasmid, pDH1. In order to make Methylomonas sp. DH-1 more attractive for metabolic engineering, 1. we have updated the genomic annotation, 2. we have reconstructed the metabolic network Methylomonas sp. DH-1, and 3. we tried to reconstruct the transcription unit for the strain. Based on this knowledgebase, we also compared the transcriptome data when grown on methane and methanol to investigate activated C1 assimilation pathways. |
