| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 봄 (04/04 ~ 04/06, 대전컨벤션센터) |
| 권호 |
43권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Inflammatory Proteinase Responsive Fluorogenic Hydrogel Lens Sensor |
| 초록 |
Hydrogels have offered numerous biological applications such as implantable scaffold in tissue engineering, and biosensors due to their several advantages; (i) high water content enabling biological reactions taken inside the gel, (ii) high stability and biocompatibility and (iii) protection from non-specific adsorptions of proteins. Meanwhile, MMP2&9 have been investigated widely because they are highly related with inflammatory diseases. Specifically, cleavage of certain peptide sequences is accompanied during enzymatic reaction of MMP2&9. Herein, we developed MMP9 responsive lens-shaped fluorogenic hydrogel using peptide probe which specifically cleavable with MMP9. In detail, vinyl group terminated peptide probe was conjugated during formation of PEGDA hydrogel. Peptide probe inside the hydrogel sensor cleaved with existence of MMP9, becoming fluorescence off-state into on-state by FRET effect. Fluorescence intensity was increased with concentration of MMP9 increased. |
| 저자 |
신무광, 강병훈, 진우석, 김명훈, 기지선, 한승민, 이형근, 함승주
|
| 소속 |
연세대 |
| 키워드 |
MMP9; hydrogel; peptide; sensor; inflammation
|
| E-Mail |
|
