| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.376 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Degradation Behavior of Chitosans with Various Degree of Acetylation |
| 초록 | Chitosan is a linear heteropolysaccharide composed of β-1,4-linked D-glucosamine (GlcN) and N-acetyl-D-glucosamine (GlcNAc) with various compositions of these two monomers.1 The chain structure and degree of acetylation (DA) of chitosan influence not only the physicochemical characteristics, but also the biodegradability and immunological activity.2 Commercially available chitosan has been obtained by deacetylation of purified chitin with concentrated alkali and high temperature treatment. However, their low solubility (thus processing difficulty) in common solvents caused by block-type distribution of the GlcNAc residues and low DA reproducibility which leads non-uniform physicochemical properties and biodegradability are still remained as some limitations.3 To overcome these problems, we synthesized DA-controlled chitosans (from 10% to 90%) by the acetylation reaction of deacetylated chitosan (DA ∼ 10%) and acetic anhydride with different ratio. The DA value of the chitosans was characterized by FT-IR and solid state 13C NMR. The prepared acetylated chitosans seem to have random-type GlcNAc residues, as evidenced by X-ray diffraction (WAXS) and solubility experiment. The degradation behavior of the acetylated chitosans was investigated in lysozyme solution which is a kind of enzymes for chitosan in the human body. The degradation rate of the chitosans was observed as follows, according to the DA values: 50% ＞ 30% ≈ 70% ＞ 90% ＞ 10% (그림 1). Fig. 1. Degradation behavior of acetylated chitosans in lysozyme solution (10 mg/L in PBS). (a) Molecular weight change (by end group analysis); (b) Weight loss (film-type). Acknowledgment This work was supported by a grant from Korea Ministry of Commerce, Industry and Energy. References 1. A. Domard and M. Rinaudo, Int. J. Biol. Macromol., 5, 49 (1983). 2. C. Chatlet, O. Damour, and A. Domard, Biomaterials, 22, 261 (2001). 3. K. Kurita, M. Kamiya, and S. I. Nishimura, Carbohydr. Polym., 16, 83 (1991). |
| 저자 | 송대근1, 오세행1, 이동신2, 배은희2, 이진호1 |
| 소속 | 1한남대, 2(주) 리젠바이오텍 |
| 키워드 | Chitosan; Degradation; Acetylation |
