| 초록 |
A novel protocol for the synthesis of HA-PLGA using HA-ADH was successfully developed for its periodontal barrier applications. The degree of HA modification with ADH could be increased up to 85 mol% in the mixed solvent of water and ethanol. Highly modified HA-ADH appeared to be soluble in DMSO with enhanced stability to enzymatic degradation by hyaluronidase. When HA-PLGA was blended with PLGA in chloroform, amphiphilic biphasic films were obtained with hydrophilic HA and hydrophobic PLGA layers. Among four different samples of a control, OSSIX™ membrane, PLGA film and HA-PLGA/PLGA film implanted to cover the calvarial critical size bone defects in SD rats, HA-PLGA/PLGA film resulted in the most effective bone regeneration followed by OSSIX™ membrane and PLGA film. The novel biphasic HA-PLGA/PLGA film will be investigated further as a periodontal barrier film for effective guided bone regeneration. |
