| 학회 |
한국화학공학회 |
| 학술대회 |
2007년 봄 (04/19 ~ 04/20, 울산 롯데호텔) |
| 권호 |
13권 1호, p.392 |
| 발표분야 |
생물화공 |
| 제목 |
Microfluidic sample preparation system for integration of micro mixer and solid phase extraction |
| 초록 |
A microfluidic cell lysis chip integrated the micro-mixer and Solid phase extraction (SPE) was developed for achieving a micro total analytical system for the analysis of cells and their constituent proteins. This work was focused on the development of miniaturized sample preparation systems where cell disruption is needed to obtain intracellular materials for analysis of protein. It consists of a magnetically actuated micro-mixer for disrupting bacterial cell of intracellular green fluorescent protein (GFP), hydrophobic valve for cell lysate manipulation, and packed porous polymerized monolith chamber for solid phase extraction (SPE) and filtering debris from cell lysate. |
| 저자 |
허윤석1, 최종현2, 박태정3, 이은주4, 홍연기5, 홍원희6, 이상엽7
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| 소속 |
1Department of Chemical and Biomolecular Eng., 2KAIST, 3Metabolic and Biomolecular Engineering National Research Lab, 4Dep. of Chemical and Biomolecular Eng., 5Dep. of Biosystems, 6Bioinformatics Research Center, 7BioProcess Engineering Research Center and Center for Ultramicrochemical Process Systems |
| 키워드 |
cell lysis; SPE; sample preparation; green fluorescent protein
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| E-Mail |
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| 원문파일 |
초록 보기 |
