| 학회 | 한국공업화학회 |
| 학술대회 | 2012년 가을 (10/31 ~ 11/02, 대전컨벤션센터) |
| 권호 | 16권 2호 |
| 발표분야 | 초청강연 |
| 제목 | MICRO- AND NANO-CONTAINERS BASED ON BIO-POLYMERS |
| 초록 | The creation of bio-polymer micro- and nano-containers that are suitable for encapsulating of biologically-active substances is important and actual problem of modern pharmacology. The inclusion of medical substances into polymer matrix allows to decrease their toxicity, obtain new pharmaceutical formulations with improved solubility in biological liquids, controlled kinetics of release, high stability at storing, low hygroscopicity and etc. [1,2]. Nano- and micro-containers based on polysaccharides that are suitable for creation pharmaceutical formulations with prolonged action have been formed. Bio-compatible and non-toxic polysaccharides (chitosan, alginates, pectines, carraginanes) were used as structural components of nano- and micro-containers. Hollow and containing biopolymer (pectin, algine acid, carboxymethylcellulose) microcapsules based on protamine enzyme were obtained. They have the diameter of 3-5 µm and the thickness of polyelectrolyte wall from 20 to 100 nm. Formed microcapsules were used as containers for effective local concentration of anti-neoplastic formulations: methanesulphonate of imatinibe and doxorubycine hydrochloride. Alginate, chitosan, pectinate and carregeenan-pectinate nanoparticles with the size 50-110 nm were obtained by the method of ionothrope gel-forming and coacervation. It is established that the size of polysaccharide nanoparticles can be regulated by changing the concentration of a polymer in reaction mixture, its molecular mass, pH and ionic strength of the solution and also by introducing the stabilizer. It is showed that high-molecular biologically active substances (bovine serum albumin, horse-radish peroxidase, pepsine) can be included in bio-polymer nanoparticles during the synthesis process. Pectinate nanoparticles with the size from 1 to 5 µm were synthesized in reversed emulsions of “water in oil” type. Strepthokinaze and fluorescent markers on the basis of CdSe/ZnS nanoparticles were incorporated for in-vivo experiments into pectinate microspheres and, for comparison, to liposomes. It is established that the release time of strepthokinaze from micro-particles can be changed in the range from 30 min to a day by changing the concentration of sewing pectinate reagent. The possibility of controlled by ultrasound release of medicinal substance is showed. Bio-compatibility of synthesized microspheres is proved in experiments on rats. There were no factors of emboli registered. Microparticles were registered mainly in heart, liver and spleen tissues. 1. S. Sundar, J. Kundu, S.C. Kindu. Biopolymeric nanoparticles // Science and technology of advanced materials. 2010. V. 11. P. 11-13. 2. G.A. Morris, M.S. Kok, S.E. Harding, G.G. Adams Polysaccharide drug delivery systems based on pectin and chitosan // Biotechnology and Generic Engineering Reviews. 2010. V. 27. P. 257-284. |
| 저자 | Vladimir Agabekov |
| 소속 | Professor |
| 키워드 | polysaccharides; nano- and microcontainers; strepthokinaze; pectinate microsheres |
