| 초록 |
Nano-sized Graphene oxide was prepared and exploited for the target specific delivery of cancer therapeutics. Hexamethylenediamine conjugated hyaluronic acid (HA-HMDA) was tethered to carboxylated NGO by amide bond formation via EDC chemistry. The HA-NGO conjugate showed enhanced stability and non-cytotoxicity in physiological conditions. Epirubicin (Epi), which is an anthracycling cancer drug, was loaded on graphitic domain of HA-NGO conjugates through π-π stacking in a basic condition and released at an acidic condition. The controlled release of Epi from the complex in acidic endosomes resulted in the effective inhibition of tumor growth. The competitive binding tests with HA preincubation revealed the target specific delivery of HA-NGO/Epi complex to CD44 positive B16F1 cells. Finally, the confocal microscopy for the co-localization of lysotracker and epirubicin at the endosome confirmed that HA-NGO/Epi complex was delivered into the cells via receptor mediated endocytosis. |
