| 학회 |
한국고분자학회 |
| 학술대회 |
2012년 봄 (04/12 ~ 04/13, 대전컨벤션센터) |
| 권호 |
37권 1호 |
| 발표분야 |
의료용고분자 부문위원회 |
| 제목 |
Preparation and Characterization of Photo Reactive Azidophenyl Hyaluronic Acid Derivative for Protein Immobilization |
| 초록 |
To immobilize proteins such as growth factor, photo reactive azidophenyl hyaluronic acid (Az-HA) derivative was synthesized. The photo sensitizing group, 4-azidoaniline was introduced to carboxyl group of hyaluronic acid using by water soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC). To confirm the synthesis was successful, FTIR and 1H NMR spectroscopy and UV absorbance spectroscopy was performed. We also checked protein immobilizing effect of Az-HA derivative using by fluorescein isothiocyanate conjugated albumin (FITC-BSA) on the micropattern. Cytotoxicity and relative cell proliferation of with Az-HA derivative was performed on 3T3-L1 fibroblast cell lines. |
| 저자 |
이형재, 박신혜, 조영민, 허윤, 김은혜, 손태일
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| 소속 |
중앙대 |
| 키워드 |
Hyaluronic acid; 4-azidoaniline; 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; Photo reactive; Protein immobilization
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| E-Mail |
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