| 학회 | 한국고분자학회 |
| 학술대회 | 2002년 봄 (04/12 ~ 04/13, 서울대학교) |
| 권호 | 27권 1호, p.3 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Tissue-Engineered Bioartificial Skin |
| 초록 | Development of Chitosan Dermal Scaffold A variety of artificial dermis, which are mainly composed of type I collagen from animal sources, have been developed for the aid of skin regeneration in the partial thickness or full thickness wound. In spite of excellent wound healing with collagen-based artificial dermis, its low structural integrity, easy degradation due to contamination on wound site, and its high cost limit its wide clinical application. We have developed tensile and porous chitosan dermal scaffold mainly composed of neutralized chitosan, whose pore sizes were controlled by freezing temperature and have improved its low cell binding capacity significantly by mixing or coating atelomeric type I collagen. We also recreated wound-healing microenvironment in chitosan/collagen dermal scaffold by supplementing various concentrations of bFGF and fibronectin, which improved the cell binding capacity of chitosan sponges in vitro and the wound healing effect in animal experiment. New Cell Isolation Method for Tissue Engineering of the Skin The proportion of epidermal stem cell determines the expansion potential and the intake of cultured epidermal autografts. Therefore, we developed epidermal cell isolation method to obtain highly enriched stem cells. Our new method showed 6.5 fold higher cell yield than Green’s method. Colony forming efficiency was 1.15% by our method, 0.95% by Green’s, 0.28% by thermolysin, and 0.83% by dispase. Consequently, the total number of colony forming cells obtainable per 1 cm2 adult foreskin was 8.8 fold higher by our method than by Green’s method. The epidermal cells isolated by our method were b1 integrin-bright cells, putative stem cells. In conclusion, our cell isolation method is advantageous over other conventional methods, which is essential to expand epidermal stem cells for tissue engineering. Self Cell Sorted Skin Equivalent (CeSSE) and Cell Preconditioning Individual cell possesses the intrinsic capacity to reconstruct tissue where they were derived. Tissue engineering could utilize this innate ability of cells. Engrafting of dermal fibroblasts and keratinocytes showed complete regeneration of human skin on the back of nude mice, whose reconstruction of basement membrane was confirmed by immunohistochemical staining of laminin5 and type IV collagen. The intake rate of the skin equivalent after grafting is very low, which may be due to the lower proportion of stem cells in the skin equivalent or the lower survival rate of the delivered cells in the wound microenvironment. The latter problem may be overcome by cell preconditioning before the skin graft. We applied equiaxial cyclic strains to the dermal fibroblasts using Flexcell-4000 system under the serum free condition with or without PDGF and/or IGF-1. Cyclic strains increased the cell density by 5 fold and fibronectin expression by 300 folds within two days. Application of cyclic strains did not induce transdifferentiation of dermal fibroblasts into myofibroblasts. In contrast, PDGF and/or IGF did induce myofibroblastic transdifferentiation. In the CeSSE on the nude mice, cell survival rate was higher in preconditioned group. Therefore the preconditioned cells may adapt or survive much better under the wound environment. |
| 저자 | 손영숙1, 박현숙2, 김천호3, 김태환, 김윤영*, 김창환, 강현주*, 이수현*, 최영주*, 임세환, 한은숙, 진용재, 한규보, 황태숙** |
| 소속 | 1원자력병원 생체조직재생연구실, 2*(주)엠티티 부설(연), 3**인하대 |
| 키워드 | |
| VOD | VOD 보기 |
