| 학회 |
한국고분자학회 |
| 학술대회 |
2003년 가을 (10/10 ~ 10/11, 부경대학교) |
| 권호 |
28권 2호, p.2 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Photo-induced Release of Plasmid from Crosslinked Nanoparticles |
| 초록 |
While viruses efficiently package and protect their genome only to release their DNA or RNA at the appropriate stage of transduction, the engineering of triggered release in synthetic DNA particles has proven challenging since protection and release are competing design goals. We report the synthesis of o-nitrobenzyl and methacrylate functionalized polyethyleneimine for condensing plasmid (4.7 kbp) into particles as small as 100 nm after radical polymerization. DNA was not extractable from cross-linked nanoparticles until photoirradiated at 365 nm (3.5 mW/cm2) for 10 min. Plasmid condensed with uncrosslinked monomers transfected COS cells. However, crosslinked DNA nanoparticles produced no transgene expression until photoactivated, either extracellularly or intracellularly. These reagents may prove useful in the generation of stable DNA nanoparticles for temporally-controlled, spatial-addressed, and metered dosing of DNA for gene transfer. |
| 저자 |
김문석1, 강길선2, 조선행1, 신병철1, 이해방1, Scott L. Diamond3
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| 소속 |
1한국화학(연), 2전북대, 3Univ. of Pennsylvania |
| 키워드 |
DNA; polyethyleneimine; Nanoparticles; Photo
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| E-Mail |
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