| 초록 |
The work describes an optimized process to highly efficient and convenient preparation in high throughput magnetic human DNA separation with chemically functionalized silica-coated magnetic nanoparticles. The effect of nanoparticle’s size and the surface’s hydrophilicity change were studied for magnetic DNA separation process, in which the optimum efficiency was explored via the function of the amino-group numbers, particle size, the amount of the nanoparticles used, and the concentration of NaCl salt. The DNA adsorption yields were high in terms of the amount of triamino-functionalized nanoparticles used, and the average particle size was 25 nm. The adsorption efficiency of aminofunctionalized nanoparticles was the 4-5 times (80-100%) higher compared to silica-coated nanoparticles only (10-20%). DNA desorption efficiency showed an optimum level of over 0.7 M of the NaCl concentration. To elucidate the agglomeration of nanoparticles after electrostatic interaction, the Guinier plots were calculated from small angle X-ray diffractions in a comparison of the results of electron diffraction TEM, and confocal laser scanning microscopy. Additionally, the direct separation of human genomic DNA was achieved from human saliva and whole blood with high efficiency. |
