| 초록 |
Acetate is an unwanted byproduct of microbial fermentation, but has a great potential as cost-effective and alternative carbon source in the production of various platform chemicals. This study demonstrates development and engineering of Escherichia coli strain for 3-hydroxypropionic acid (3-HP) production from acetate. Combinatorial strategies of metabolic engineering including the disruption of acetyl-CoA competing pathways, manipulation of glyoxylate shunt, improvement of cofactor (i.e., NADPH) supply through the TCA cycle, and redirection of the gluconeogenesis pathway have been employed. Both growing and resting cells could produce 3-HP from acetate with the highest yield 0.6 mol/mol, about higher than the theoretical maximum |
