| 초록 |
Determining transcriptional and translational regulatory elements in GC-rich Streptomyces genomes is essential to elucidating the complex regulatory networks that govern secondary metabolite biosynthetic gene cluster (BGC) expression. However, information about such regulatory elements has been limited for Streptomyces genomes. To address this limitation, high-quality genome sequences of antibiotic-producing Streptomyces strains were completed, which contains several thousands of newly annotated genes. This genome information provides fundamental references to integrate multiple genome-scale data types, including dRNA-Seq, RNA-Seq, Term-Seq, and ribosome profiling. Data integration results in the precise determination of thousands of transcription start sites which reveal transcriptional and translational regulatory elements. This comprehensive genetic information provides a versatile genetic resource for rational engineering of secondary metabolite BGCs in Streptomyces. |
