| 학회 |
한국고분자학회 |
| 학술대회 |
2016년 봄 (04/06 ~ 04/08, 대전컨벤션센터) |
| 권호 |
41권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Poly (2-Hydroxyethyl Methacrylate)-b-Poly (L-Lysine) Cationic Hybrid Materials for Non-Viral Gene Delivery in NIH 3T3 Mouse Embryonic Fibroblasts |
| 초록 |
In order to develop efficient and nontoxic gene delivery vectors, a series of biocompatible block copolymers, poly[(2-hydroxyethyl methacrylate)40-block-(L-lysine)n] (n= 40, 80, 120, 150), are prepared by combining an atom transfer radical polymerization of 2-hydroxyethyl Methacrylate with a ring-opening polymerization of NƐ-(carbobenzoxy)-L-lysine N-carboxyanhydride. The block copolymers are successfully condensed with plasmid DNA (pDNA) into nanosized (＜200 nm) polyplexes. As a representative sample, p- (HEMA)40-b-p(lys)150 is utilized to confirm the effective cellular and nuclear uptake of pDNA. The polymer/pDNA polyplexes exhibit very low cytotoxicity and enhanced transfection activity by being easily taken up into mouse embryonic fibroblast cell line (NIH 3T3). Thus, the chimeric block copolymers provide a means for developing versatile nonviral gene vectors harboring the ideal requirements of low cytotoxicity, good stability, and high transfection efficiency for gene therapy. |
| 저자 |
Rajendrakumar Santhosh Kalash1, Saji Uthaman2, Renjith P. Johnson3, Kamali Manickavasagam Lekshmi4, Cherukula Kondareddy1, Il Kim2, 박인규3
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| 소속 |
1Department of Biomedical Science and BK21 PLUS Center for Creative Biomedical Scientists at Chonnam National Univ., 2Chonnam National Univ. Medical School, 3Gwangju 501-746, 4South Korea |
| 키워드 |
gene delivery; transfection; fibroblast cell line; nontoxic; plasmid DNA
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| E-Mail |
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