화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2016년 가을 (10/26 ~ 10/28, 제주국제컨벤션센터(ICCJEJU))
권호 20권 2호
발표분야 한국생산기술연구원의 기능성 소재 최신 연구동향
제목 Production of Lactic Acid from  Various Biomass
초록 Recently, oil-shortage and environmental pollution have caused increasing needs of producing biochemicals using non-food and waste biomass to replace petroleum based chemicals. In this study, we constructed yeast strains (Saccharomyces cerevisiae) producing lactic acid which is used to be a monomer for producing polylactic acids (PLA) with fermentable sugars derived from rice straw, waste of pine tree and food wastes. Fermentable sugars derived from the each biomass were prepared using acid hydrolysis and ammonia pretreatment. We constructed a S. cerevisiae harboring a plasmid containing a codon-optimized lactate dehydrogease (LDH) gene derived from Bos taurus and selected an optimal promoter to increase expression of the LDH. We produced 10.6 g/L of lactic acid and 13.0 g/L of ethanol with 40 g/L of fermentable sugars derived from the rice straw under flask fermentation.
저자 유병조
소속 한국생산기술(연)
키워드 biomass; lactic acid; lactate dehydrogease; Saxxharomyces cerevisiae
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