| 초록 |
In this study, we fabricated an exogenous growth factor delivery platform as coacervate-coated poly (lactic-co-glycolic acid) (PLGA) nanofiber. The coacervate is a self-assembled complex which was consisted of poly(ethylene arginyl aspartate diglyceride) polycation, heparin, and cargo growth factors. We investigate coating efficiency of coacervate on the PLGA nanofiber through surface analysis and cellular bioactivity of released VEGF and TGF-β3 from coacervate-coated PLGA nanofiber (Coa-Dual) via proliferation of human dermal fibroblast and tubular formation of human umbilical vein endothelial cells. The efficacy of Coa-Dual group in mouse skin flap survival was evaluated by comparing the results from several groups. The Coa-Dual group not only decreased necrosis area of skin flap but also enhanced blood perfusion as compared with the other groups. Moreover, Coa-Dual group observed in histologically well-composed skin appendage and significantly enhanced angiogenesis in wound bed. |
