| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 가을 (10/26 ~ 10/28, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
20권 2호 |
| 발표분야 |
나노_포스터 |
| 제목 |
Global DNA methylation detection via plasmonic coupling-enhanced surface enhanced Raman scattering (SERS) |
| 초록 |
Cytosine methylation in DNA refers to genetic changes without mutation of actual genetic nucleotide sequence. DNA methylation in certain genes is deeply involved in several fatal diseases, but current methods for detecting methylation entails high cost and large sample size. Here, we developed a surface-enhanced Raman spectroscopy (SERS)-based platform for detecting global DNA methylation state. The methylation sites were detected with anti-mCpG immunogold colloids, and the signal was enhanced with plasmon coupling effect. The DNA samples were absorbed onto positively-charged surface of silver nanowire (AgNW) and immunogold colloids were attached on methylated sites. The detection limits for global methylated DNA on the silver nanowires were 18 fg/mL. |
| 저자 |
전명진, nguyenhunganh, 심상준
|
| 소속 |
고려대 |
| 키워드 |
DNA methylation; plasmon coupling; SERS
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| E-Mail |
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