| 학회 |
한국화학공학회 |
| 학술대회 |
2003년 봄 (04/25 ~ 04/26, 순천대학교) |
| 권호 |
9권 1호, p.1083 |
| 발표분야 |
재료 |
| 제목 |
High sensitive sonsor system using immobilized oligopeptide and polystyrene beads |
| 초록 |
A high-sensitive sensor that uses an oligopeptide and a piezoelectric quartz crystal microbalance(QCM) was developed for detection of low molecular weight compounds under aqueous condition. Porphyrin 5,10,15,20-tetrakis(N-methylpyridium-4-yl)-21H,23H-porphine (TMpyP) and a porphyrin binding peptide (HASYS) obtained from a phage library were used as the target compound and the target binding material, respectively. A self-assembled monolayer (SAM) of the peptide on the gold electrode surface of QCM (9 MHz, AT-cut) was formed to capture the porphyrin. To achieve ng/ml level detection, we developed a novel sensitization technique using latex beads on which the same porphyrin binding peptide was immobilized. The detection sensitivity of the peptide-immobilized QCM sensor could be improved by addition of the peptide-immobilized latex beads to the detection system. The detection limit was 10 ng/ml of TMpyP. The mass sensitization was achieved by adsorption of the latex beads onto the QCM surface via sandwich binding of porphyrin. |
| 저자 |
송성훈1, C. Nakamura2, J. Miyake2, 장상목1
|
| 소속 |
1동아대, 2일본 산업기술융합(연) |
| 키워드 |
QCM; Oligopeptide; porphyrin; polystyrene beads
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| E-Mail |
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