| 초록 |
Development in understanding the physicochemical properties of nanoparticles (NPs) is essential for rational and optimized applications. Electrochemical technique is one of the powerful tools for characterization of NPs. Here, an electrochemical biosensor was developed for detection of mycobacterium tuberculosis (Mtb) antigen (CFP-10) using Fe3O4@Ag/GQD modified glassy carbon electrode (GCE) as a sensing platform and gold nanoparticles (AuNPs) as a label for signal amplification. A sandwich type immunoassay was prepared by immobilizing antibody 1 (Ab1), and AuNPs functionalized with antibody 2 (Ab2−AuNPs) on Fe3O4@Ag/GQD modified GCE. The immunosensor showed a wide linear range (0.005 - 500 µg/mL) with a limit of detection (LOD, S/N = 3) reaching 0.33 ng/mL. The result showed good performance with a high selectivity, and simple operation, can be easily extended to other pathogenic bacteria detection. |
