| 초록 |
Single-cell nanoencapsulation (SCNE) demands cytocompatible materials and processes, to ensure the maintenance of cell viability, and prefers facile post-functionalization of the cytoprotective shells. This work proposes a hydrogen bonding-based layer-by-layer (LbL) self-assembly of eggshell membrane hydrolysate (ESMH) and tannic acid (TA) as a cytocompatible, and post-functionalizable, strategy applied to SCNE. As a proof-of-demonstration, individual Saccharomyces cerevisiae cells have been encapsulated, by the LbL multilayers of ESMH and TA, in an astonishingly cytocompatible manner (viability after SCNE: >99%). In addition to the cytoprotection capability, the ESMH/TA shell proves functionalizable, for example, including the bioinspired silicification for formation of organic/inorganic double-layered shells. |
