화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터)
권호 28권 1호, p.1252
발표분야 [주제 12] 화학공학일반(부문위원회 발표)
제목 Droplet based microfluidic system for rapid antimicrobial susceptibility testing at a single cell level.
초록 We demonstrate the droplet based microfluidic platform for analysis of antimicrobial susceptibility at single cell level. First, we make the single cell encapsulated microdroplet generator integrated with linear concentration gradient to control the composition of antibiotic mixture. Using this strategy, we tested gentamicin against gram negative bacteria (i.e. E.coli O157:H7 ) to determine the minimum inhibitory concentration(MIC). Screening the growth profile of cells in droplets having different concentration of gentamicin resulted in MIC determination in only 4-6 h using our device. Additionally, we compare the minimum inhibitory concentration result from bulk system using an Erlenmeyer flask. This result show that our platform has the great potential to be used for antimicrobial susceptibility testing (AST) with other bacteria (i.e. gram-positive bacteria) and antibiotics.
저자 오서연, 김재성, 이병진, 정헌호, 이창수
소속 충남대
키워드 생물화공(Biochemical Engineering)
E-Mail rhadum@cnu.ac.kr
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