| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.375 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Preparation and enzymatic degradation of chitin and chitosan nanofibers |
| 초록 | 1. Introduction Chitin is the principal structural polysaccharide of the arthropods and the second most abundant polysaccharide, next to cellulose. However, it has received much less attention because of its poor solubility, while considerable interest has been devoted to biomaterials based on its amino-derivative chitosan. Until now, many researchers have investigated chitin or chitosan, as one of candidate materials for biomedical applications because it has several distinctive biological properties including good biocompatibility, biodegradability, and wound healing effect. In practice, it has been used in various fields, such as cosmetics, medical materials for human health, and food additives. The aim of this study is to develop a nonwoven-type wound dressings composed of the electrospun chitin (or chitosan) nanofibers. 2. Experimental Chitin solutions were prepared by dissolving the irradiated chitin powders in HFIP for 20days. Concentration of chitin solution for electrospinning was 5% by weight. In the electrospinning process, a high electric potential was applied to a droplet of chitin solution at the tip of a syringe needle. The electrospun chitin nanofibers were collected on a target drum. For deacetylation, chitin nanofiber matrix was treated with a 40% NaOH aqueous solution at 100℃ for 150min. Degree of deacetylation was calculated by NMR spectrum. In vitro degradation, chitin and chitosan matrix was incubated in 4mg/ml of egg-white lysozyme solution in PBSsolution (pH 7.2) at 37℃ for 0~30 days. The extent of degradation was expressed as the percentage of the dryweight of the remainder after immersion against initial weight. 3. Results and Discussion Chitin nanofiber matrix was produced by the electrospinning process. Within the deacetylation for 150min at 100℃, chitin matrix was transformed into chitosan matrix with DD=85%. This structural transformation from chitin to chitosan was confirmed by FT-IR and WAXD. Figure 1 shows the SEM micrographs of chitin and deacetylated chitin (chitosan) nanofiber matrix Chitin readily undergoes degradation in PBS containing lysozyme. Degree of weight loss of each specimen in PBS increased with time of incubation, and specimen was almost degraded around 30days. Fig. 1. SEM micrographs of chitin and deacetylated chitin (chitosan). |
| 저자 | 이성원1, 임정남1, 박원호1, 민병무2 |
| 소속 | 1충남대, 2서울대 |
| 키워드 | chitin; chitosan; degradation; electrospinning |
