| 학회 |
한국고분자학회 |
| 학술대회 |
2012년 가을 (10/11 ~ 10/12, 창원컨벤션센터) |
| 권호 |
37권 2호 |
| 발표분야 |
대학원생 구두발표 |
| 제목 |
Branched-PEI conjugated Cationic Polymer for Gene Delivery with Low Cytotoxicity and High Transfection Efficiency |
| 초록 |
The aim of this study was to investigate the effect of branched-PEI(bPEI) conjugated cationic polymer such as chitosan on in vitro effect of transfection efficiency and cytotoxicity at varying cell lines. O-carboxymethyl chitosan(CMCh) was prepared by chemical reaction chitosan with mono-chloroacetic acid to C6-OH posion of low molecular weight water soluble chitosan(LMWSC). MTT assay indicated that OCMPEI complex was significantly less toxic than bPEI. The transfection efficiency in cancer cell and normal cell of the OCMPEI copolymers were evaluated. Results of transfection efficiency showed that the highest transfection efficiency was observed in OCMPEI complex with pDNA or siRNA(16:1,w/w). It is expected that the OCMPEI complex may be a good carrier for gene delivery. This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology(2012-0001621) |
| 저자 |
남정표, 김영민, 장미경, 나재운
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| 소속 |
순천대 |
| 키워드 |
bPEI; chitosan; pDNA; siRNA;
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| E-Mail |
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