| 학회 |
한국화학공학회 |
| 학술대회 |
2007년 가을 (10/26 ~ 10/27, 한국과학기술원) |
| 권호 |
13권 2호, p.1722 |
| 발표분야 |
생물화공 |
| 제목 |
Lipase-catalyzed synthesis of glucose fatty acid ester using ionic liquids mixtures |
| 초록 |
The low solubility of sugars has hampered the lipase-catalyzed synthesis of fatty acid sugar esters in organic solvents and ionic liquids (ILs), because several solvents that are able to effectively dissolve sugars are detrimental to enzymes. In order to prepare a high concentration of sugars in ILs, we have developed a new procedure that entails mixing an aqueous sugar solution into ILs followed by removal of the water from the solution. In this work, lipase-catalyzed synthesis of 6-O-lauroyl-D-glucose in ILs mixtures was investigated by using supersaturated glucose solution. Although the activity of Novozym 435 in a 1-butyl-3-methylimidazolium trifluoromethanesulfonate ([Bmim][TfO]) and 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide mixture (1:1 v/v) was somewhat lower than that in pure [Bmim][TfO] which showed the highest enzyme activity, the stability of Novozym 435 was significantly increased. Specifically, the activity of Novozym 435 was increased from 1.1 to 2.9 μmol/min/g by using supersaturated glucose solution in this mixture, compared with reaction using saturated solution. |
| 저자 |
힙민응웬1, 이상현2, 하성호2, 장우진2, 구윤모1
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| 소속 |
1인하대, 2ERC 초정밀 분리기술 연구센터 |
| 키워드 |
sugar ester; lipase; ionic liquid; mixture; supersaturation
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| 원문파일 |
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