| 초록 |
The development of an efficient platform for the growth and neuronal differentiation of stem cells is crucial for autologous cell therapy and tissue engineering. In this study, we describe the use of highly uniform graphene platforms, which is advantageous for the differentiation of hMSCs into neurons. We suppose that graphene regulates the interactions at cell-substrate or cell–cell interfaces, consequently promoting the neurogenesis as well as the outgrowth of neurites. We also demonstrated that the differentiated neurons on graphene are notably sensitive to external ion stimulation, and their neuronal properties can be maintained even after detaching and re-seeding onto a normal cell culture substrate, suggesting the enhanced maturity of resulting neuronal cells. Thus, we conclude that monolayer graphene is capable of regulating the growth and neural differentiation, which would provide new insight and strategy for autologous stem cell therapy and tissue engineering. |
