| 학회 | 한국화학공학회 |
| 학술대회 | 2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 | 18권 1호, p.500 |
| 발표분야 | 생물화공 |
| 제목 | A lipid-supported gene-networking hydrogel as an artificial chromatin nucleus mimicry |
| 초록 | Recent biomimetic engineering have principally studied a cellular engineering system mimicking its natural multi-components representing each organelle. For instance, a mimicry of a nuclear pore transporter, a virus-like particle for drug delivery or a tunable immune system has been developed in the field. Inspired by these advances, we have demonstrated a gene-networking hydrogel(G-net-gel) as a biomimetics of an organized chromatin in the cell nucleus. Spherodical G-net-gels were manufactured onto the superhydrophobic layer using both simple lithography and DNA engineering methods. The G-net-gel was optimized in various parameters to improve the enhanced protein levels in in vitro. The stable gene cross-networks were fused into lipid molecules, finally leading to lipid-supported g-net-gel networks resembling the living nucleus which be capable of producing the higher level of messenger RNAs as compared with solution phase vector. The artificial chromatin nucleus biomimicry would be a substitute to the conventional gene therapy, provided that it replace malfunctional but pre-located nucleus. |
| 저자 | 배선주, 남경호, 엄숭호 |
| 소속 | 성균관대 |
| 키워드 | biomimetic engineering; gene-networking hydrogel; artificial nucleus mimicry |
| 원문파일 | 초록 보기 |
