| 학회 |
한국공업화학회 |
| 학술대회 |
2005년 봄 (05/13 ~ 05/14, 충남대학교) |
| 권호 |
9권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Efficient expression system for the production of heterologous polyketides in Streptomyces venezuelae |
| 초록 |
The polyketide synthase (PKS) gene cluster responsible for tylactone biosynthesis from Streptomyces fradiae was expressed in a heterologous host, S. venezuelae. We constructed the engineered S. venezuelae mutant in which the PKS related to the biosynthesis of the native polyketides is deleted, and developed a two-vector system for heterologous expression of the five genes comprising the tylosin PKS gene cluster. Interestingly, the S. venezuelae transformants produced not only tylactone but its glycosylated derivatives, desoaminyl tylactone. The deoxysugar desosamine is synthesized by the products of desosamine biosynthetic genes in S. venezuelae. This result demonstrates that the glycosyltransferase (Des VII) is aglycone-flexible and attaches desosamine to tylactone, a 16-membered ring macrolactone. |
| 저자 |
정원석1, 이상길1, 홍성중1, 박수진2, 정순정2, 최차용1, 윤여준2
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| 소속 |
1서울대, 2이화여자대 |
| 키워드 |
polyketide; streptomyces; tylosin
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| E-Mail |
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