| 학회 |
한국재료학회 |
| 학술대회 |
2014년 봄 (05/15 ~ 05/16, 창원컨벤션센터) |
| 권호 |
20권 1호 |
| 발표분야 |
D. 바이오 재료(Biomaterials) |
| 제목 |
Chemically-Conjugated Bone Morphogenetic Protein-2 and Vitamin D-TGF on porous PCL/BCP-Collagen scaffolds for enhance bone regeneration |
| 초록 |
In this study, porous polycaprolactone (PCL)-biphasic calcium phosphate (BCP) was immobilized with Collagen, Bone morphology protein-2 (BMP-2) and VitaminD-TGF (VitD-TGF) successfully to improve the bone regeneration. The surface of PCL/BCP scaffolds was aminolyzed by 1,6-hexamethylenediamine to introduce amino groups onto their surface. Then Collagen, BMP-2 and VitD-TGF were further immobilized on the aminolyzed porous PCL/BCP via EDC/NHS crosslinking agent. The pore size of scaffolds was 200-300 µm which was suitable for bone in-growth. After immobilization, the pre-osteoblast cell viability and proliferation were improved remarkably confirmed by MTT assay, Live-dead assay and immunofluorescence. The in vivo study will be carried out by implanting the porous PCL/BCP-Collagen, PCL/BCP-Collagen-BMP-2 and PCL/BCP-Collagen-VitD –TGF to the skulls of rats. The present results provided more evidence that functionalizing PCL/BCP with BMP-2 and VitD-TGF may be a feasible way to improve the interaction between cells and also , PCL/BCP-Collagen-BMP-2 and PCL/BCP-Collagen-VitD –TGF scaffolds could be a potential candidate for bone regeneration. |
| 저자 |
Myeong Jin Song1, Nguyen Thuy Ba Linh2, Young Ki Min3, Byong Taek Lee4
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| 소속 |
1Department of Regenerative Medicine, 2Institute of Tissue Regeneration, 3College of Medicine, 4Soonchunhyang Univ. |
| 키워드 |
PCL-BCP; Collagen; Bone morphology protein-2; VitaminD-TGF; immobilization.
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| E-Mail |
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