| 학회 |
한국재료학회 |
| 학술대회 |
2013년 가을 (11/06 ~ 11/08, 제주롯데호텔) |
| 권호 |
19권 2호 |
| 발표분야 |
D. 바이오재료(Biomaterials) |
| 제목 |
Effects of recombinant human bone morphogenetic protein-2 on in vivo bone regeneration of fibrous BCP-PCL/GEL composite |
| 초록 |
To confirm effect of recombinant human bone morphogenetic protein-2(rhBMP-2) for bone regeneration, rhBMP-2 loaded biphasic calcium phosphate(BCP)-polycaprolactone(PCL)/gelain(GEL) composite fibrous scaffolds were fabricated successfully using electrospinning method. Compared with BCP-PCL/GEL scaffold, the rhBMP-2 loaded BCP-PCL/GEL scaffold shows improved biocompatibility, cell proliferation and cell adhesion behavior evaluated by in-vitro studies. The BCP-PCL/GEL scaffolds with rhBMP-2 or without were implanted in rat skull defects for 4 and 8 weeks to evaluate the detailed biological response physiological condition. In vivo bone regeneration was compared by evaluating the degrees of new bone formations using micro-CT scanning, and histological staining using hematoxylin & eosine / Masson’s trichrome staining method. The defect implanted with electrospun mat containing rhBMP-2 demonstrated highest amount of bone tissue regeneration among the samples. This result suggests that rhBMP-2 loaded BCP–PCL/GEL membranes could be considered as potential bone tissue engineering applications. |
| 저자 |
Bo-ram Kim1, Nguyen Thuy Ba Linh2, Hyoung-Seok Kim3, Young-Ki Min1, Byong-Taek Lee4
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| 소속 |
1Department of Regenerative medicine, 2College of Medicine, 3Soonchunhyang Univ., 4Institute of Tissue Regeneration |
| 키워드 |
electrospinning; polycaprolactone; gelatin; BCP; rhBMP-2; bone tissue engineering; bone regeneration
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| E-Mail |
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