| 학회 | 한국재료학회 |
| 학술대회 | 2007년 가을 (11/02 ~ 11/02, 성균관대학교) |
| 권호 | 13권 2호 |
| 발표분야 | 나노생체재료 |
| 제목 | Integrated Biocompatible Nano-Biosensor-Chip |
| 초록 | Recently it has been critical issues a new paradigm of nano-bioscience, especially about nanobiosensor, neurobiosensor, neuroelectronic devices, neurochip, ion-channel for next generation medical. For practical nanobiosensor chip using nanostructure device, it is important meaning to devetic nanopatterning with biocompatible materials by soft nanolithograpgy technology in fuelop biomimndamental science of the bioengineering area. I have been tried to develop biocompatible materials based nanopatterning, self-assembly array, implantable nanobiosensor and nanobioactuator to address challenging problem in nanobioscience. Micro/nanopatternings of biomolecules such as DNA, protein and cells have proven useful as high-throughput screening tools in proteomics, genomics and the identification of new pharmaceutical compounds. The universal patterning techniques for producing submicrometer patterns are microcontact printing, x-ray interference lithography, nanoimprint lithography and capillary lithography. A critical point for the development of biomolecule nanopatterning is avoided the nonspecific absorption among biomolecules onto surface. In this paper, I present the nanometrics geometry of a well-oriented nanowell (ONW) array derived from nanofabrication technology which can easily be employed for digital detection with a high S/N ratio, miniaturization, integrated assays and single molecule analysis. In this geometry, most of the area of the Au electrode was covered with the blocking layer, and only a nanosized gold surface becomes exposed to the open space above the ONW. We fabricated the self-organized nanopatterning of hydrogel as a platform of biomolecular nanoarry using soft capillary lithography. Ultraviolet curable mold consisting of functionalized polyurethane with acrylate group was fabricated. We also present a strong specific antibody-antigen interaction on a functional lipid-membrane vesicle (liposome, FLVs) modified gold surface using ONW array metrics. The interaction is described by electrochemical measurerment, SPR, QCM, AFM. Additionally, we present simple soft lithographic methods for patterning supported lipid bilayer (SLB) membranes onto a surface and inside microfluidic channels. The patterned copolymer surfaces resisted nonspecific adsorption of lipid vesicles allowing for adsorption of the lipid bilayers on the exposed regions of two dimensional surfaces and inside microfluidic channels in comparison to glass control. We believe these findings can be related to various liposome applications such as drug delivery system, electrochemical or biosensors and nano scale membrane function studies. |
| 저자 | HeaYeon Lee1, Tomoji Kawai2 |
| 소속 | 1ISIR-SANEKN, 2Osaka Univ. |
| 키워드 | Nano-Biosensor-Chip; Nanowell array; |
