| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
3-Hydroxypropionic acid Tolerance in Klebsiella pneumoniae: The Role of Transcriptional Regulator YieP and the Acid Transporter YohJK. |
| 초록 |
Acid tolerance is an important issue for industrial production of chemicals, such as 3-hydroxypropionic acid (3-HP). In Escherichia coli, the transcription factor, YieP, was reported to regulate yohJK, genes encoding putative 3-HP exporter(s). The deletion of YieP could greatly improve the 3-HP tolerance via overexpression of yohJK. In this study, similar phenomenon was observed in Klebsiella pneumoniae, an important host for 3-HP production.Protein comparison showed that YieP and two putative 3-HP transporters (YohJK1 and YohJK2) are present K. pneumoniae. The deletion of YieP improved 3-HP tolerance in K. pneumoniae. Moreover, transcription analysis shown that YieP negatively regulates YohJK1 but not YohJK2, suggesting that YohJK1 is the ‘true’ 3-HP exporter in K. pneumoniae. Overexpression of YohJK1 could improve 3-HP production in the 3-HP stressful condition. |
| 저자 |
NGUYEN VO PHU THUAN, Huichang Ryu, 박성훈
|
| 소속 |
Ulsan National Institute of Science and Technology / Pusan National Univ. |
| 키워드 |
Klebsiella pneumonia J2B; 3-hydroxypropionic acid; yieP; yohJK
|
| E-Mail |
|
