| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 |
21권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Promoter engineering for improved B12 production in Pseudomonas denitrificans ATCC 13867 |
| 초록 |
Coenzyme B12 is a cofactor for glycerol dehydratase, an essential enzyme in biological synthesis of 3-HP. There are two pathways for B12 biosynthesis, one is oxygen-dependent and the other one is oxygen-independent. Pseudomonas denitrificans can de novo synthesize B12 by oxygen-dependent pathway. However, its B12 biosynthesis is not sufficient. The presence of riboswitches which sense B12 and repress the transcription of B12 operons restricts the B12 biosynthesis in P. denitrificans. In this study, we eliminated the riboswitch regulation and improved B12 gene expression by systematically modifying promoter regions of B12 operons in P. denitrificans. The promoter engineering could improve B12 biosynthesis in P. denitrificans. Further improvement of B12 biosynthesis are under studying. |
| 저자 |
NGUYEN VO PHU THUAN1, 박성훈2
|
| 소속 |
1UNIST / PNU, 2UNIST |
| 키워드 |
coenzyme B12; riboswitch; Pseudomonas denitrificans; promoter engineering
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| E-Mail |
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