| 초록 |
Angiogenesis is a critical multistep process in development and physiology. Recent studies show that stem cells are involved in maturation of angiogenesis. However, the exact role of stem cell on angiogenesis is not fully understood. We studied the effects of two major conditions on the sprouting of cell-based spheroids. First, we changed the ratio of endothelial cell (EC) to the mesenchymal stem cell (MSC), which consist spheroids, to 1:1 and 4:1 to verify the stem cell effects on EC sprouting in the 3D collagen matrix. Second, we applied angiogenic growth factors, such as vascular-endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF), both individually and in combination to study the degree of vessel development in the matrix. Our results show highest level of sprouting when all three growth factors were treated on EC/MSC ratio of 4:1. This study can be applied to cell therapy for angiogenesis in vivo. |
