| 학회 |
한국공업화학회 |
| 학술대회 |
2006년 가을 (11/10 ~ 11/11, 경희대학교(수원캠퍼스)) |
| 권호 |
10권 2호 |
| 발표분야 |
생물공학 |
| 제목 |
Optimization of One-Bead One-Compound (OBOC) Library Synthesis for Kinase Substrate Screening by Using MALDI-TOF MS |
| 초록 |
We have developed the one-bead one-compound (OBOC) combinatorial library method for the screening of enzyme substrate specificity. In the OBOC library , each of the resin beads carries not only the full-length peptide product but also small amounts of sequence-specific N-terminated ones.. The sequence of the full-length peptide is determined by using MALDI-TOF MS, which measures the mass differences between the adjacent amino acid added peptides and the N-terminated peptides. We optimized the condition to control the amounts of N-terminated peptide products to give the maximum purity of the full-length peptide. |
| 저자 |
김미라, 신동식, 최정현, 김병기, 이윤식
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| 소속 |
서울대 |
| 키워드 |
peptide library; MALDI-TOF
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| E-Mail |
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