| 초록 |
Gellan Gum (GG) has been applied as an effective hydrogel for tissue engineering, especially for cartilage regeneration. However, pristine GG is not enough to provide a biological environment. Demineralized bone particles (DBPs) are known for a biomaterial which comprise of collagen, proteoglycan matrix and bone morphogenetic protein (BMP). Herein, a composite of DBPs and GG was fabricated to increase cell viability, cell adhesion, chondrogenesis in vitro, and enhance articular cartilage regeneration in vivo. DBPs/GG hydrogel was characterized by measuring water uptake, swelling ratio, porosity, ATR-FTIR and Bio-LV SEM. Cell viability was measured by MTT assay, sulfated glycosaminoglycan (sGAG) content and mRNA expression analysis was examined for chondrogenesis. Overall, DBPs/GG hydrogel appears to be a promising substrate for cartilage regeneration. This research was supported by a grant of the Korea Health Technology R&D Project through KHIDI (HI15C2996) |
