| 초록 |
In recent, biomarkers from liquid biopsy have potentially emerged for cancer diagnosis. Because of cancer heterogeneity, it is important that their identification and quantification should proceed in various systematic ways. Among the diverse methods, localized surface plasmon resonance (LSPR)-based optical nanobiosensors are considered one of the most powerful tools in the fields of biosensors. LSPR possesses the specific characteristics of metallic nanostructured materials, such as noble metal nanoparticles, which can be excited by irradiation with incident photons and is resonant with the collective oscillations of conduction electrons at a specific wavelength. Herein, we have developed immuno-nanoplasmonics composed of systemic aptamer-integrated nanoprobes to measure and assess the sensing efficiency for target biomarkers. Immuno-nanoplasmonics consist of uniform plasmonic nanoparticles that are sequentially functionalized and chemisorbed aptamers. To confirm the sensing capability of immuno-nanoplasmonics regarding specificity, selectivity, and stability for the biomarker, the LSPR signal was measured and verified under biological meida. Single-nanoparticle scattering analysis by the nanoscattering spectrum imaging analysis (NSSIA) system was used in this approach. Sensing with single nanoparticles has received significant attention due to its ability to greatly increase the sensitivity of analyte sensing, that is to say, the absolute detection limit (numbers of analyte molecules per nanoparticle) is dramatically reduced. Moreover, since only a few nanoparticles need to be monitored at a time, the required sample volume is significantly lower than for ensemble methods. Using these properties, there have been several demonstrations of bioimaging and biosensing on the scattering of a single nanoparticle |
