| 학회 |
한국재료학회 |
| 학술대회 |
2018년 가을 (11/07 ~ 11/09, 여수 디오션리조트) |
| 권호 |
24권 2호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
Endothelial Cell Response on In vitro Generated Extracellular Matrix |
| 초록 |
Endothelial cells is the main cell type associated with the development of functional vascular networks. Biomaterials used as blood vessel grafts and as scaffolds for blood vessel tissue engineering should allow attachment, proliferation and functionalization of vascular cells. In this study, response of cow pulmonary endothelial cells (CPAE) on in vitro generated extracellular matrix (ECM) derived from bone marrow stem cells (BMSC) and fibroblast cells (L929) was observed. 104cells/ml of cell density BMSC and L929 were cultured on the PCL(Polycaprolactone) membrane in varying amounts of serum supplement (10%, 20%, 30%) for one week. The plates were then decellularized using either SDS, Triton-X 100, or water to yield the corresponding ECM. Biomolecules such as nucleic acids (DNA/RNA), collagen and sulfated glycosaminoglycans (sGAG) in the plates were compared before and after decellularization. Endothelial cell response was observed through confocal microscopy and cell proliferation assay of CPAE seeded on the ECM containing plates. Results show that increasing the serum content significantly affect the ECM synthesis of both BMSC and L929. Biomolecule content of decellularized ECM is also highly dependent on the decellularization solution used. Endothelial cells also respond differently depending on the source and composition of the extracellular matrix. In this study the corresponding endothelial cell response on extracellular matrix soured from different cells was investigated through optimization of in vitro ECM synthesis and decellularization process. Information gathered in this study can be used in the development of ECM based vascular grafts and in vitro models of endothelial cell response. |
| 저자 |
Minji Choi1, Byong taek Lee2
|
| 소속 |
1Regenerative Medicine in College of Medicine Soonchunhyang Univ., 2Institute of Tissue Regeneration |
| 키워드 |
endothelial cell; BMSC; L929; ECM; SDS; Triton-X100; decellularization. |
| E-Mail |
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