| 초록 |
We propose aminated polysorbate 80 (P80-NH2) as a new non-viral synthetic gene delivery material with sufficient gene transfection efficiency and reduced cytotoxicity. Aminated polysorbate 80 was synthesized from polysorbate 80, a polymer of branched poly (ethylene oxide) with the amine structure of spermine, through the replacement of the hydrophilic groups (Figure 1). In addition, the hydrophobic group of polysorbate 80 plays a role of strong interaction with the cellular membranes, and thereby enhance a cellular internalization of DNA. Spermine containing the amine group is existed as polycation under physiological pH, which is natural polyamine that stabilizes the helical structure of nucleic acids29 |
