| 초록 |
Recently, bone marrow stem cells (BMSCs) and adipose delivered stem cells (ADSCs) are widely used for tissue engineering and regenerative medicine. During stem cell culture, we use trypsin-EDTA for subculture, however major side effect of trypsin-EDTA is invasive to not only adhesive protein but also membrane protein. In this study, we fabricated thermo-responsive poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofiber via electrospinning for rapidly cell recovery. The temperature-responsive poly(N-isopropylacrylamide) (PIPAAm) was grafted onto PHBV nanofiber surface by electron beam irradiation for non-invasive cell recovery. PHBV nanofiber and PIPAAm-grafted PHBV nanofiber surfaces were chracterized by scanning electron microscopy (SEM), image analysis, attenuated total reflection-infrared (ATR-FTIR) spectroscopy and water contact angle goniometer. |
