초록 |
The influence of culture temperature as a stressful condition on cell growth and physiological adaptation in microalga, Tetraselmis sp., was investigated. Cells were grown under fluorescent lamps at 80 µE•m-2•s-1 in modified f/2 medium, with aeration of 2% CO2 in bubble columns at 10℃, 20℃ and 30℃. The optimal cultivation temperature to produce biomass was 20℃ with a specific growth rate of 1.03 d-1 and FAMEs content of 12%. Under 30℃, the major FAs were palmitic acid oleic acid and linoleic acid, whereas linolenic acid, stearidonic acid and eicosenoic acid were shown to be the dominant FAs at 10℃. It supposed that growth rate and unsaturation of FAs were influenced by temperature change. This results will help to understand the control of cellular metabolism under various cultivation temperature and the interaction of related metabolism in microalgae. |