| 학회 |
한국고분자학회 |
| 학술대회 |
2021년 가을 (10/20 ~ 10/22, 경주컨벤션센터) |
| 권호 |
46권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Colorimetric detection of influenza virus by Avidin-Biotinylated GNP complex with Layer-by-Layer signal amplification |
| 초록 |
Unpredictable pandemic caused by viruses raised the need for rapid detection which plays a crucial role in the efficient management on site. Herein, we designed colorimetric immunoassay for influenza virus detection with layer-by-layer signal amplification based on avidin & biotinylated Gold Nanoparticles(bGNPs) complex. To accomplish this system, we identified interaction with bGNPs and virus captured by antibody on a microtiter plate. In the presence of virus, red color appears and the visibility is proportionate to the number of Avidin-bGNPs. And peroxidase mimicking property of GNPs that catalyzes the chromogenic substrates with H2O2 offers another amplification that leads to enhanced intensity and the color becomes blue. Finally, we verified the naked-eye detection of influenza virus within 2h, shorter than that of ELISA. Thus, the designed system not only suggests alternative way to conventional colorimetric immunoassays but serve as promising tool for field diagnostic tests. |
| 저자 |
정은지, 박근선, 김진영, 박채원, 이소정, 함승주
|
| 소속 |
연세대 |
| 키워드 |
Gold nanoparticles; Avidin-biotin bonding; Layer by layer; Signal amplification; Enzyme mimicking; Biosensor; Naked eye detection; virus detection
|
| E-Mail |
|
