| 학회 |
한국고분자학회 |
| 학술대회 |
2019년 가을 (10/09 ~ 10/11, 제주컨벤션센터) |
| 권호 |
44권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Efficient self-assembled microRNA delivery system using PEGylated polycationic polymer and cholesterol-conjugated microRNA |
| 초록 |
MicroRNA (miR), a key molecule involved in endogenous RNA interference, is a promising therapeutic agent. In vivo delivery of miR, however, is a major factor limiting its application because its polyanionic nature and vulnerability to breakdown make delivery of miR to targeted lesions difficult. To overcome these challenges, we developed a self-assembled miR delivery system consisting of cholesterol-conjugated miR and polyethylene glycol(PEG) grafted polyethylene imine (PEI). Nanosized complexes of miR with PEI, which protected miR and its delivery into targeted lesions in vivo, were synthesized by PEG grafting. The hydrophobicity of cholesterol improved the structural stability of the complex, preventing the loss of miR. Here, we report the preparation of this self-assembled complex. We examined the delivery efficiency and validated the therapeutic efficacy of the complex. In conclusion, our miR delivery system shows considerable potential for effective in vivo delivery of miR. |
| 저자 |
문병걸1, 장은지2, 한승민3, 손혜영1, 최유나1, 허용민1, 함승주1
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| 소속 |
1연세대, 2Novomics Co. Ltd., 3The Univ. of Arizona |
| 키워드 |
self-assembled miRNA delivery; RNA interference; cholesterol conjugation; polycationic polymer; cancer therapy
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| E-Mail |
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