| 초록 |
Developing synthetic biological devices to allow the noninvasive control of cell fate and function, in vivo can potentially revolutionize the field of regenerative medicine. To address this unmet need, we designed an artificial biological “switch” that consists of three parts: (1) the electromagnetic perceptive gene (EPG). (2) mouse myoblast C2C12 Cell line with pcDNA3.1-EPG_RFP and (3) iron oxide nanoparticles (IONPs). Our group has recently cloned the EPG from the Kryptopterus bicirrhis (glass catfish). The EPG gene encodes a putative membrane-associated protein that responds to electromagnetic fields (EMFs). This gene’s primary mechanism of action is to raise the intracellular calcium ion levels or change in flux through EMF stimulation. Furthermore, we developed a system for the remote regulation of Ca2+ (i.e., intracellular calcium ion concentration) using IONPs under EMFs. The results demonstrated that the EPG can be used as a molecular Ca2+ switch to express target EPG proteins. IONPs can maintain intracellular Ca2+ levels. This technology has the potential for controlled gene expression, drug delivery, drug developments and treatment of muscle disease. |
